Organizing Section: Glaucoma Program Number: 1791 Presentation Time: 11:00 AM–11:15 AM Restoration of trabecular meshwork cellularity and function following iPSC transplantation in vivo
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چکیده
These abstracts are licensed under a Creative Commons Attribution-NonCommercial-No Derivatives 4.0 International License. Go to http://iovs.arvojournals.org/ to access the versions of record. Purpose: Primary open angle glaucoma is frequently associated with loss and dysfunction of trabecular meshwork (TM) cells. The purpose of this study was to determine if restoration of TM cellularity, using TM cells derived from induced pluripotent stem cells (iPSC), can lead to lasting functional rescue of the TM in a genetic mouse model of the disease. Methods: Mouse iPSC were differentiated into TM-like cells (iPSC-TM) and injected into the anterior chamber of MYOC Y437H mice (N=22) as described previously. Controls included MYOC Y437H mice receiving sham injections (N=16) or fibroblasts (N=8) as well as age matched wild type mice (N=20). Intraocular pressure (IOP) was measured by rebound tonometry and aqueous humor outflow facility by direct cannulation. 12 weeks after transplantation retinal ganglion cell (RGC) and TM cellularity was assessed by immunohistochemistry and gene expression patterns in the TM was determined using gene expression arrays.
منابع مشابه
Restoration of Aqueous Humor Outflow Following Transplantation of iPSC-Derived Trabecular Meshwork Cells in a Transgenic Mouse Model of Glaucoma.
Purpose Primary open-angle glaucoma (POAG) is particularly common in older individuals and associated with pathologic degeneration of the trabecular meshwork (TM). We have shown previously that transplantation of induced pluripotent stem cell (iPSC) derived TM cells restores aqueous humor dynamics in young transgenic mice expressing a pathogenic form of human myocilin (Tg-MYOCY437H). This study...
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تاریخ انتشار 2016